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为了探究栽培基质对银耳Tremella fuciformis子实体中氨基酸合成的影响，采用代谢组学和蛋白质组学比较分析以代料（70%棉籽壳和30%麸皮组成）和青冈木为栽培基质栽培的银耳子实体的氨基酸含量，并结合关联分析探究栽培基质影响银耳氨基酸合成的机制。结果表明，代料栽培银耳TF1子实体的蛋白质和氨基酸含量以及生长速度显著高于青冈木栽培银耳TY3。通过液质联谱分析，筛选出显著差异代谢物488种，其中氨基酸、肽类及其类似物占比最高，利用蛋白质组学方法筛选出668个差异表达蛋白质。对代谢组与蛋白质组数据进行关联分析，发现银耳色氨酸、酪氨酸、赖氨酸等氨基酸合成通路和戊糖磷酸途径的调控网络，进一步验证得出代料栽培银耳通过增加NADPH促进氨基酸合成，导致其氨基酸含量显著高于青冈木基质生长银耳。研究有助于探究栽培基质调控银耳氨基酸合成的机制，为发展银耳产业优质高效栽培模式提供理论依据。
Abstract：In order to explore the regulatory effect of culture substrates on amino acid synthesis in the fruiting body of Tremella fuciformis, metabolomics and proteomics were applied in this study. Two kinds of substrates(a mixed substrate made of wheat bran and cottonseed hull and a Cyclobalanopsis substrate) were used to for the investigation, which revealed a result that the total contents of proteins and amino acids of T. fuciformis grown on the mixed substrate were significantly higher than those grown on the Cyclobalanopsis substrate. A total of 488 metabolites with significant differences in quantity between the two substrates were identified by LC-MS/MS, in which amino acids and peptides including their similar substances accounted for the highest proportion. Moreover, a total of 1 685 proteins were identified by using proteomics and 668 differentially expressed proteins were screened out. Correlation analysis of metabolomic and proteomic data revealed a presence of the regulatory network on the amino acid synthesis pathway, such as tryptophan, tyrosine and lysine and pentose phosphate pathways. Our study demonstrated, furthermore, an increment of NADPH content in the T. fuciformis grown on the mixed substrate, which led to the higher amino acid content of T. fuciformis than that of Cyclobalanopsis substrate. The above results obtained at the present study should help to understand the regulatory mechanism of amino acid synthesis under the different cultivation substrates in T. fuciformis and provide a theoretical basis for developing a high-quality and efficient cultivation model as well.
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基本信息:
DOI：10.13341/j.jfr.2024.1841
中图分类号:S567.34
引用信息:
[1]许瀛引,张仕林,罗定平等.基于代谢组和蛋白组联合分析栽培基质对银耳氨基酸合成的影响[J].菌物研究,2025,23(03):231-239.DOI:10.13341/j.jfr.2024.1841.
基金信息:
国家自然科学基金项目（32402653）; 通江县食用菌科研合作项目（SCTJ-2023001）; 四川省农科院重点科研项目（1+9KJGG007）